Standardization of antigenemia and qpcr cut-off values in whole blood for the detection of cytomegalovirus disease in hiv patients

Abstract INTRODUCTION: We defined the cut-off values of the antigenemia and cytomegalovirus (CMV) DNA tests in HIV/AIDS patients to identify CMV disease. METHODS: A total of 97 samples from 68 patients with and without CMV disease were analyzed by viral DNA detection and antigenemia assay. RESULTS: Qualitative and quantitative results significantly differed between assays. The cut-off values for the antigenemia and qPCR assays were 1.5 positive cells/200,000 leukocytes and 3.715 log/mL, respectively. CONCLUSIONS: Antigenemia and qPCR are suitable for monitoring CMV disease in HIV patients, however, the threshold values should be determined within the centers where the patients are monitored.

Análise eletromiográfica de músculos da coluna vertebral durante a marcha em diferentes formas de carregar o bebê / Electromyographic analysis of the spinal muscles during the gait in different ways to carry a baby

Introdução: A forma de carregar o bebê pode causar desequilíbrios em músculos relacionados à coluna vertebral. Objetivos: Investigar a atividade elétrica dos músculos trapézio fibras descendentes e eretores lombares durante a marcha na simulação de carregar um bebê. Métodos: 20 voluntárias, destras e saudáveis participaram das coletas durante a marcha em esteira na simulação do ato de carregar o bebê em diferentes formas: 1) posição horizontal; 2) posição vertical; 3) suporte tipo "canguru" e 4) suporte tipo "sling". Os resultados eletromiográficos foram analisados pela análise da intensidade do sinal eletromiográfico dos músculos trapézio fibras descendentes e eretores espinhais lombares bilateralmente. Resultados: A ANOVA de dois fatores indicou que a forma de carregar (F(4,59)=17,1 p<0,001) e lado (F(1, 59)=89,6 p<0,001) afetaram a intensidade da atividade do músculo eretor espinhal e também a forma de carregar (F(4, 59)=6,4 p<0,001) e lado (F(1, 59)=59,9 p<0,001) do músculo trapézio fibras descendentes. Conclusões: O suporte canguru gerou menor ativação em músculos espinhais durante a quando comparado a outras situações.

Introduction: The way to carry the baby may cause imbalances in muscles related to the spine. Objectives: To investigate the electrical activity of the muscles trapezius descending fibers and lumbar erector during the simulation of carrying a baby. Methods: 20 volunteers right-handed and healthy participate of the study during walking on a treadmill to simulate the act of carrying the baby in different ways: 1) horizontal position; 2) vertical position; 3) Support Type "kangaroo" and 4) Support Type "sling". The EMG results were analyzed by analysis of the intensity of the electromyographic signal of the muscles trapezius fibers descendants and spinal lumbar erector bilaterally. Results: A two-way ANOVA indicated that the way of loading (F(4, 59)=17.1 p <0,001) and next (F(1, 59)=89.6 p <0.001) affected the intensity of the activity spinal erector muscles and also the way of loading (F(4, 59)=6.4 p <0.001) and next (F(1, 59)=59.9 p <0.001) of the trapezius muscle fibers descendants. Conclusions: Kangaroo support generated less activation in spinal muscles during when compared to other situations.